Components

• Convenient pack sizes
• Multi-purpose use in hybridizations
• Easy-to-use powder makes fast, inexpensive solutions

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Application

Suitable for:
• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.