Components

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Application

Suitable for:
• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

Features and Benefits

• Multi-purpose use in hybridizations
• Easily reconstituted and diluted to any concentration needed

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.