Components

Sigma’s DirectLoad^™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol.

Application

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

General description

Sigma’s DirectLoad^™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Features and Benefits

• Ready-to-load
• Easy-to-use
• Popular band sizes

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC