Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.

Features and Benefits

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

General description

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Packaging

1, 5 mg in glass bottle

Preparation Note

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Quantity

One mg DNA is approx. 20 A₂₆₀ units.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.