Components

Gel loading buffer contains 0.05% bromophenol blue , 40% sucrose, 0.1M EDTA (pH 8.0) and 0.5% SDS.

Application

Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.

General description

Gel loading buffer is used as a tracking dye during electrophoresis. The dye has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample before loading.

Other Notes

If crystals form, dissolve them by heating the solution to 65C for 10 minutes.