PRODUCT NUMBER:

MW: 6000-6500 (Reduced/Heated)

Buffer: 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 100 μM DTT

Form: Lyophilized

Storage: -20ºC or lower

Purity: Greater than 95% by SDS-PAGE

Biological Significance: S-100 is a low molecular weight, cytoplasmic, calcium binding protein. It is traditionally found in the brain, heart and muscle tissue. S-100 has two subunits (alpha-chain and beta-chain) and exists in several different forms. The alpha-alpha homodimer is referred to in literature as either the S-100αα or S-100a(0), the beta-beta homodimer is referred to in literature as S-100b and the alpha-beta heterodimer is referred to in literature as S-100a. ART purifies a mixture of all forms (S-100), S-100αα (<5% beta chain contamination by Western Blot), and S-100b (<10% alpha chain contamination by Western Blot).

References: Neurochemical Research 13, 561-565 (1988); J Biol Chem 261, 8204-8212 (1986); FEBS Lett 148, 231-234 (1982); Biochem Biophys ACTA 494, (1977), 222-232; J Biol Chem 246. 6276-6283 (1971)

Prepared from tissue collected in the contiguous United States from animals under 30 months of age at a USDA inspected facility. Animals were inspected ante and post mortem and found free of infectious diseases.

Country of Origin: Bovine brain was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research use only.