Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.

Product Specifications
Bicine;
N,N-bis(2-hydroxyethyl)glycine;
di(hydroxyethyl)glycine;
N,N- bis(hydroxyethyl)aminoacetic acid

ULTRA PURE GRADE

Formula: C₆H₁₃NO₄

MW: 163.17 g/mol

PubChem Chemical ID: 8761

Storage/Handling: Store at room temperature.