Description

The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO vector, which is compatible with the cloning of blunt-end PCR fragments.  Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit.  Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:

• ccdB gene for positive selection
• EcoR I site flanking the PCR product insertion site for easy excision of inserts
• Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
• T7 promoter/priming site for in vitro RNA transcription and sequencing
• T7, T3, and M13 forward and reverse primer sites for sequencing

The TOPO XL-2 Complete PCR Cloning Kit includes:

• TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
• Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>100X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
• PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
• One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.