用于膜片钳研究的多通道灌流给药系统

次级离子通道前沿的研究

用于膜片钳研究的多通道灌流给药系统

The Dynaflow® Resolve 设备是您现有膜片钳设置的配件，能够以的速度，控制和灵活性提供解决方案交换。 该系统可以记录任何离子通道电流，使用任何细胞类型，任何化合物和任何实验。

优势

真正的多功能性 适用于任何细胞类型，任何膜片钳配置以及任何类型的离子通道，可以进行从从单细胞通道记录到二次筛选的多方面研究。 复杂的灌流设计方案

快速溶液交换（低毫秒范围）能够达到高效地分析快速动力学。

低溶液的消耗并且可重复使用的芯片。

可以达到消耗150 μl的长时间实验，大限度地减少化学溶液和缓冲液的消耗。

可以达到大优化，有效的安quan药理学

可以进行长时间的实验和长时间给药。 针对粘性化合物进行了优化设计，并设计适用于累积剂量反应。

大化的数据质量

每芯片可以操作大量的细胞。 每个芯片多个化学物质分析。 对于每个化合物可以达到wanquan剂量反应。 Gigaseal 可以记录达到卓越的实验控制。

设备

 

该设备包括一个带控制器和操纵杆的电动扫描平台，驱动微流控芯片流动的注射泵以及 Dynaflow® Commander 软件。 它可以与大多数倒置显微镜合用。

1 – Dynaflow® Resolve 芯片

Dynaflow® Resolve 芯片具有16个微通道，并且由一个玻璃微型芯片和一个塑料外壳可以组成微槽和记录室。 该芯片可重复使用，并且用于降低“粘性化合物”的非特异性结合的可能，并通过降低低化合物消耗从而改善效率成本。

2 -带控制器和操纵杆的电动扫描平台

机动扫描台用于自动移动芯片位置。 它包括一个扫描台的控制器和一个操纵杆，并且可以由 Dynaflow® Commander 软件控制。

3 – 注射泵

配有一个易于安装的注射泵并且专门设计用于形成 Dynaflow® Resolve 芯片中的流柱。

4 – Dynaflow® Commander 软件

软件确保和控制扫描台的运动以及相关预编程。 它将精度的进行溶液交换与所获取实时数据以及进行标记同步。 基于 Windows 的 Dynaflow® Commander 软件可以quan面地掌控实验，包括扫描流程，给药时间和瞬时的实验流程改变。

• Structural basis of α-scorpion toxin action on Nav channels View paper >>

• Insensitivity to Pain upon Adult-Onset Deletion of Nav1.7 or Its Blockade with Selective Inhibitors View paper >>

• Pharmacological Activation of hERG Potassium Channels in Congenital Long QT Syndrome 2: Activator Compound ICA-105574 and its Effects on Mutant hERG Potassium Channels in Long QT Syndrome 2 View paper >>

• Galantamine is not a positive allosteric modulator of human α4β2 or α7 nicotinic acetylcholine receptors View paper >>

• Discovery of GluN2A-Selective NMDA Receptor Positive Allosteric Modulators (PAMs): Tuning Deactivation Kinetics via Structure-Based Design View paper >>

• Probing Structure and Function of Ion Channels Using Limited Proteolysis and Microfluidics View paper

• Glycine/Serine Polymorphism at Position 38 Influences KCNE1 Subunit’s Modulatory Actions on Rapid and Slow Delayed Rectifier K+ Currents View paper

• Kinetics of drug interaction with the Kv11.1 potassium channel View paper

• Effect of cholesterol depletion on the pore dilation of TRPV1 View paper

• Sub-anesthetic concentrations of (R,S)-ketamine metabolites inhibit acetylcholine-evoked currents in α7 nicotinic acetylcholine receptors View paper

• A method for bidirectional solution exchange—“Liquid bullet” applications of acetylcholine to α7 nicotinic receptors View paper

• Alternatively spliced domains interact to regulate BK potassium channel gating View paper

• AF-353, a novel, potent and orally bioavailable P2X3/P2X2/3 receptor antagonist View paper

• Arrangement of Kv1 α subunits dictates sensitivity to tetraethylammonium View paper

• Novel Alpha-7 Nicotinic Acetylcholine Receptor Agonists Containing a Urea Moiety: Identification and Characterization of the Potent, Selective, and Orally Efficacious Agonist 1-[6-(4-Fluorophenyl)pyridin-3-yl]-3-(4-piperidin-1-ylbutyl) Urea (SEN34625/WYE-103914) View paper

• Telithromycin blocks neuromuscular transmission and inhibits nAChR currents in vitro View paper

• MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity View paper

• Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1 View paper

• Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells View paper

• Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade View paper

• Controlling Desensitized States in Ligand-Receptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols View paper

• A Biohybrid Dynamic Random Access Memory View paper

• An isoflurane- and alcohol-insensitive mutant GABAA receptor 1 subunit with near normal apparent affinity for GABA: characterization in heterologous systems and production of knock-in mice View paper

• Blocking Characteristics of hERG, hNav1.5, and hKvLQT1/ hminK after Administration of the Novel Anti-Arrhythmic Compound AZD7009 View paper

• Microfluidic device for creating gradients View paper

• A chemical waveform synthesizer View paper

• Microfluidic technologies in drug discovery View paper

• Patch clamp electrophysiology steps up a gear View paper

• Microfluidic Gradient Generating Device for Pharmacological Profiling View paper

• Blocking characteristics of hKv1.5 and hKv4.3/hKChIP2.2 after administration of the novel antiarrhythmic compound AZD7009 View paper

• A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes View paper

• Stabilization of High-Resistance Seals in Patch-Clamp Recordings by Laminar Flow View paper

• Automated Electrophysiology: High Throughput of Art View paper

• A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions View paper

• Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells View paper