鼠源WNT-2 cDNA基因克隆
英文名称： Mouse WNT-2 cDNA gene clone / ORF gene clone
型号:null    产品货号： MG50420-M
价格:请致电：010-57128832,18610462672
品牌: 北京

官方网址：http://www.sinobiological.cn/WNT-2-cDNA-Clone-g-4608.html
 
WNT2 cDNA Clone Datasheet
 
 
 
 
 
 
 
WNT2 cDNA基因克隆产品信息
 
  
 NCBI登录号:  NM_023653.5 
 
  
cDNA基因长度: 
 		  
1066
 		 
 
 别名:   
Irp; Mirp; Wnt-2; Wnt2a; Int1l1; 2610510E18Rik; Wnt2
 		 
 
 种属: Mouse 
  
 
WNT2 cDNA基因克隆产品描述
 
  
  
cDNA基因描述: 
 		  
ORF Clone of Mus musculus wingless-related MMTV integration site 2 DNA.
 		 
 
 克隆载体: Vector pMD18-T  
 
 序列信息: Same as Gene Bank Ref. ID sequence with the nucleotide (66-82) deletion mutation and the point mutation 318 G/A not causing the amino acid variation. 
  
 
WNT2 cDNA基因克隆邮寄信息
 
 
     
  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
    2.  
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
    3.  
 
 
WNT2 cDNA基因克隆使用方法
 
 
     
  1. Remove the marked edge in the center of the spot.
    2.  
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
    3.  
  3. Transfer the tube to a heating block at 95oC for 15–30 min .
    4.  
  4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap, the sample approximately 60 times.
    5.  
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
    6.  
 
 
WNT2 cDNA基因克隆序列以及质控指标
 
基因序列:
 
 
1 ATGAACGTCC CTCTCGGTGG AATCTGGCTC TGGCTCCCTC TGCTCTTGAC CTGGCTCACC
61 CCTGAGGTAC ATGAGAGCTA CAGGTGGCTC CTCCAGGGTG ATGTGTGACA ATGTGCCAGG
121 CCTGGTGAGC CGGCAGCGTC AGCTGTGCCA CCGACACCCA GATGTGATGC GTGCCATTGG
181 CCTGGGTGTG GCTGAGTGGA CTGCAGAGTG CCAACACCAG TTCCGCCAGC ATCGCTGGAA
241 CTGCAACACC CTGGACAGAG ATCACAGCCT CTTTGGCCGG GTCCTCCTCC GAAGTAGTCG
301 AGAATCGGCC TTTGTTTACG CCATCTCTTC AGCTGGCGTT GTATTTGCCA TCACCAGGGC
361 CTGTAGCCAA GGAGAATTAA AGTCCTGCTC CTGTGATCCA AAGAAGAAAG GAAGTGCCAA
421 GGACAGCAAA GGCACCTTCG ACTGGGGTGG CTGCAGTGAC AATATTGACT ACGGGATCAA
481 GTTTGCCCGT GCCTTTGTAG ATGCCAAGGA GAGGAAAGGC AAGGATGCCA GAGCCCTGAT
541 GAACCTTCAC AACAACAGAG CTGGAAGGAA GGCTGTAAAG CGCTTCTTGA AACAAGAATG
601 CAAGTGTCAT GGTGTGAGTG GCTCCTGTAC TCTGAGGACA TGCTGGCTGG CCATGGCTGA
661 CTTCAGGAAA ACAGGCGACT ATCTCTGGAG GAAGTACAAT GGGGCCATCC AGGTAGTCAT
721 GAACCAGGAT GGCACTGGCT TCACTGTAGC CAATAAGAGG TTTAAGAAGC CAACGAAAAA
781 TGACCTCGTG TATTTTGAGA ATTCTCCAGA CTACTGTATC AGGGACCGAG AGGCAGGCTC
841 CCTGGGTACA GCGGGCCGTG TGTGCAACTT GACTTCCCGA GGCATGGACA GCTGCGAAGT
901 TATGTGTTGT GGGAGAGGCT ATGACACATC CCACGTCACC CGGATGACCA AGTGTGAGTG
961 TAAATTCCAC TGGTGCTGTG CCGTGCGCTG TCAGGACTGC CTGGAGGCCC TGGACGTGCA
1021 CACATGCAAG GCCCCCAAGA GTGCCGACTG GGCGACGCCT ACATGA
 
 
 
质控指标:cDNA sequencing results is available upon request.