LRCH4/LRRN1,富含亮氨酸重复家庭蛋白4抗体

产品编号HZ-9330R
英文名称LRCH4/LRRN1
中文名称富含亮氨酸重复家庭蛋白4抗体
别 名Leucine rich repeat neuronal protein 4; Leucine rich repeats and calponin homology (CH) domain containing 4; Leucine-rich neuronal protein; LRN; LRRN1; LRRN4; PP14183; SAP25; Sin3 associated polypeptide, 25kDa; LRCH4_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Rabbit,
LRCH4/LRRN1,富含亮氨酸重复家庭蛋白4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量73kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human LRCH4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
LRCH4/LRRN1,富含亮氨酸重复家庭蛋白4抗体PubMedPubMed
产品介绍background:
Members of the leucine-rich repeat family include LRCH1, LRCH2, LRCH3 and LRCH4. All family members contain one calponin-homology domain and nine leucine-rich repeats. LRCH4 (leucine-rich repeats and calponin homology (CH) domain containing 4), also known as LRN, LRRN1, LRRN4 or SAP25, is a 683 amino acid protein that belongs to the leucine-rich repeat family. The carboxy-terminus of LRCH4 may act as a membrane anchor between cells, while the amino-terminus contains the leucine-rich domains, which is thought to be involved in ligand binding. The calponin homology (CH) domain is suggested to confer Actin binding to a variety of cytoskeletal and signaling molecules. The gene encoding LRCH4 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome.

Subcellular Location:
Cytoplasm.

Similarity:
Contains 1 CH (calponin-homology) domain.
Contains 9 LRR (leucine-rich) repeats.

Gene ID:
4034

LRCH4/LRRN1,富含亮氨酸重复家庭蛋白4抗体Database links:
Entrez Gene: 4034 Human
Entrez Gene: 231798 Mouse
Entrez Gene: 100516002 Pig
Entrez Gene: 360779 Rat
SwissProt: O75427 Human
SwissProt: Q921G6 Mouse
Unigene: 125742 Human
Unigene: 200763 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-LRCH4 Polyclonal Antibody, Unconjugated(HZ-9330R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining