Pumilio 1,PUM1蛋白抗体

产品编号HZ-11218R
英文名称Pumilio 1
中文名称PUM1蛋白抗体
别 名HSPUM; KIAA0099; PUM 1; PUM L1; PUM1; PUM1_HUMAN; PUMH 1; PUMH; PUMH1; Pumilio homolog 1; Pumilio-1; Pumilio1; PUML 1; PUML1.
说 明 书0.1ml 0.2ml
研究领域发育生物学 干细胞 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
Pumilio 1,PUM1蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量126kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Pumilio 1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Pumilio 1,PUM1蛋白抗体PubMedPubMed
产品介绍background:
Pumilio 1, also known as PUM1, PUMH1 (Pumilio homolog 1), HSPUM, PUMH or PUML1, is a homolog of the Drosophila Pumilio protein and belongs to the PUF family. The PUF family is comprised of evolutionarily conserved proteins that contain a C-terminal RNA binding domain made up of eight highly conserved tandem repeats. PUF proteins function as sequence-specific RNA-binding proteins and bind NREs (nanos response elements) in the 3’-untranslated regions of target mRNAs. They play an important role mediating mRNA stabilization and repressing translation. Pumilio 1 is a typical PUF protein expressed in fetal tissues as well as adult stomach, kidney, intestine, muscle, brain and heart tissues. Pumilio 1 localizes to the cytoplasm and is believed to participate in cell fate, cell development, cell differentiation and maintenance of somatic stem cells.

Function:
Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).

Subunit:
Binds in a sequence-specific manner to the 3'-UTR of some mRNAs.

Subcellular Location:
Cytoplasm (Probable).

Tissue Specificity:
Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested.

Pumilio 1,PUM1蛋白抗体Similarity:
Contains 1 PUM-HD domain.
Contains 8 pumilio repeats.

Database links:
UniProtKB/Swiss-Prot: Q14671.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍

background: Pumilio 1, also known as PUM1, PUMH1 (Pumilio homolog 1), HSPUM, PUMH or PUML1, is a homolog of the Drosophila Pumilio protein and belongs to the PUF family. The PUF family is comprised of evolutionarily conserved proteins that contain a C-terminal RNA binding domain made up of eight highly conserved tandem repeats. PUF proteins function as sequence-specific RNA-binding proteins and bind NREs (nanos response elements) in the 3’-untranslated regions of target mRNAs. They play an important role mediating mRNA stabilization and repressing translation. Pumilio 1 is a typical PUF protein expressed in fetal tissues as well as adult stomach, kidney, intestine, muscle, brain and heart tissues. Pumilio 1 localizes to the cytoplasm and is believed to participate in cell fate, cell development, cell differentiation and maintenance of somatic stem cells. Function: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity).  Subunit: Binds in a sequence-specific manner to the 3'-UTR of some mRNAs.  Subcellular Location: Cytoplasm (Probable).  Tissue Specificity: Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. Similarity: Contains 1 PUM-HD domain.  Contains 8 pumilio repeats.  Database links: UniProtKB/Swiss-Prot: Q14671.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Pumilio 1 Polyclonal Antibody, Unconjugated(HZ-11218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining