p47/NSFL1C,血小板47蛋白抗体

产品编号HZ-9902R
英文名称p47/NSFL1C
中文名称血小板47蛋白抗体
别 名DJ776F14.1; MGC3347; NSF1C_HUMAN; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; NSFL1C; OTTHUMP00000029972; OTTHUMP00000029974; p47; P47 protein; P97 cofactor p47; SHP1 homolog; UBX domain protein 2C; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C.
说 明 书0.1ml 0.2ml
研究领域心血管 细胞生物 信号转导 生长因子和***
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Sheep,
p47/NSFL1C,血小板47蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Pleckstrin/p47
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
p47/NSFL1C,血小板47蛋白抗体PubMedPubMed
产品介绍background:
Platelet P47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

Function:
Major protein kinase C substrate of platelets.

Subcellular Location:
Nucleus. Golgi apparatus > Golgi stack. Chromosome. Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks.

Tissue Specificity:
Major protein kinase C substrate of platelets.

Post-translational modifications:
Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.

Similarity:
Contains 1 DEP domain.
Contains 2 PH domains.

Database links:
Entrez Gene: 5341 Human
Entrez Gene: 56193 Mouse
Omim: 173570 Human
SwissProt: Q6Q308 Dog
SwissProt: P08567 Human
SwissProt: Q9JHK5 Mouse
Unigene: 468840 Human
Unigene: 98232 Mouse

p47/NSFL1C,血小板47蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.