FRG2B,FRG2B蛋白抗体
英文名称： Anti-FRG2B antibody
型号:null    抗体货号： HZ-9549R
价格:请致电：010-57128832,18610462672
品牌: 中国/美国    产品商标: hzbscience

FRG2B,FRG2B蛋白抗体

产品编号HZ-9549R
英文名称FRG2B
中文名称FRG2B蛋白抗体
别 名FRG2A; FRG2B; FRG2C; FRG2B_HUMAN; FSHD region gene 2 protein family member B; HSA10-FRG2; Protein FRG2-like-1.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 发育生物学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
FRG2B,FRG2B蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量31kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human FRG2B
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
FRG2B,FRG2B蛋白抗体PubMedPubMed
产品介绍background:
Chromosome 3 is made up of about 214 million bases encoding over 1,100 genes. Notably, there is a chemokine receptor gene cluster and a variety of human cancer related loci on chromosome 3. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Key tumor suppressing genes on chromosome 3 encode apoptosis mediator RASSF1, cell migration regulator HYAL1 and angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the FRG2 family.

Database links:
Entrez Gene: 441581 Human
SwissProt: Q96QU4 Human
Unigene: 690471 Human

FRG2B,FRG2B蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

t subunit band is either a proteolytic product of the polypeptide or the product of an alternative translation start site. O-GlcNAc transferase is expressed as multiple transcripts that are present in different amounts in various human tissues, with the highest levels of expression in pancreas. Immunofluorescence of human cells expressing rat O-GlcNAc transferase indicated that it is present in both the nucleus and cytosol. HeLa cells expressing O-GlcNAc transferase do not survive well during prolonged incubations, suggesting that this protein may be toxic to the cells.

Function:
Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues.
Isoform 2, the mitochondrial isoform (mOGT), is cytotoxic and triggers apoptosis in several cell types including INS1, an insulinoma cell line.

Subunit:
Heterotrimer; consists of one 78 kDa subunit and two 110 kDa subunits dimerized via TPR repeats 6 and 7. Interacts (via TPR repeats 6 and 7) with ATXN10 (By similarity). Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, HCFC1, PPP1CC and ACTB. Component of a THAP1/THAP3-HCFC1-OGT complex. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts directly with HCFC1; the interaction O-glycosylates HCFC1, regulates its proteolytic processing and transcriptional activity and, in turn, stabilizes OGT in the nucleus. Interacts (via TPRs 1-6) with SIN3A; the interaction mediates transcriptional repression in parallel with histone deacetylase.

O-GlcNAc transferase,O位N-乙酰葡萄糖胺OGT抗体Subcellular Location:
Isoform 2: Mitochondrion. Membrane. Note=Associates with the mitochondrial inner membrane. Isoform 3: Cytoplasm. Nucleus. Cell membrane. Note=Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1. After insulin induction, translocated from the nucleus to the cell membrane via phophatidylinisotide binding. Colocalizes with AKT1 at the plasma membrane. Isoform 4: Cytoplasm. Nucleus.

Tissue Specificity:
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

Post-translational modifications:
Ubiquitinated, leading to its proteasomal degradation.

DISEASE:
Note=Regulation of OGT activity and altered O-GlcNAcylations are implicated in diabetes and Alzheimer disease. O-GlcNAcylation of AKT1 affects insulin signaling and, possibly diabetes. Reduced O-GlcNAcylations and resulting increased phosphorylations of MAPT/TAU are observed in Alzheimer disease (AD) brain cerebrum.

Similarity:
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats.

Gene ID:
8473

Database links:
Entrez Gene: 532053 Cow
Entrez Gene: 8473 Human
Entrez Gene: 108155 Mouse
Entrez Gene: 664652 Pig
Entrez Gene: 26295 Rat
Omim: 300255 Human
SwissProt: O15294 Human
SwissProt: Q8CGY8 Mouse
SwissProt: Q27HV0 Pig
SwissProt: P56558 Rat
Unigene: 405410 Human
Unigene: 259191 Mouse
Unigene: 491168 Mouse
Unigene: 82705 Rat

O-GlcNAc transferase,O位N-乙酰葡萄糖胺OGT抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.