Thymosin beta 4,胸腺素β4抗体

产品编号HZ-9870R
英文名称Thymosin beta 4
中文名称胸腺素β4抗体
别 名Tβ4; FX; Hematopoietic system regulatory peptide; Prothymosin beta 4; PTMB 4; PTMB4; Seraspenide; T beta 4; T beta-4; TB4X; THYB 4; Thyb4; Thymosin beta 4; Thymosin beta 4 X chromosome; Thymosin beta 4 X linked; TMSB 4; TMSB4; TMSB4X; TYB4_HUMAN.
说 明 书0.1ml 0.2ml
研究领域心血管 信号转导 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
Thymosin beta 4,胸腺素β4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量4.8kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Thymosin beta 4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Thymosin beta 4,胸腺素β4抗体PubMedPubMed
产品介绍background:
Proteins in the thymosin beta family are highly conserved polar peptides that bind monomeric Actin and thereby inhibit Actin polymerization. These proteins act as the main intracellular G-Actin sequestering peptides. The most abundant thymosin beta family member in mammalian cells and tissues is thymosin beta-4 (Tbeta-4), also designated Seraspenide. T beta-4 participates in several cellular events including cancerogenesis, apoptosis, angiogenesis, blood coagulation and would healing. Specifically, T beta-4 promotes cell migration and adhesion, accelerates wound healing and reduces inflammation, and becomes upregulated in a wide variety of human carcinomas. Due to the effects of T beta-4 in these events, it may become a protein of significant biological and pharmaceutical relevance.

Function:
Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.
Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Originally found in thymus but it is widely distributed in many tissues.

Similarity:
Belongs to the thymosin beta family.

Gene ID:
7114

Database links:
Entrez Gene: 7114 Human
Entrez Gene: 19241 Mouse
Entrez Gene: 81814 Rat
Omim: 300159 Human
SwissProt: P62327 Horse
SwissProt: P62328 Human
SwissProt: P20065 Mouse
SwissProt: P62329 Rat
SwissProt: Q0P5V6 Rat
Unigene: 437277 Human
Unigene: 142729 Mouse
Unigene: 409203 Mouse
Unigene: 168067 Rat
Unigene: 2605 Rat

Thymosin beta 4,胸腺素β4抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thymosin beta 4) Polyclonal Antibody, Unconjugated (HZ-9870R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.