Firefly Luciferase,荧光素酶抗体

产品编号HZ-8539R
英文名称Firefly Luciferase
中文名称荧光素酶抗体
别 名Firefly Luciferase; ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Firefly,
Firefly Luciferase,荧光素酶抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量61kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from Firefly Luciferase
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Firefly Luciferase,荧光素酶抗体PubMedPubMed
产品介绍background:
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

Function:
Produces green light with a wavelength of 562 nm.

Subcellular Location:
Peroxisome.

Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.

Database links:
SwissProt: P08659 Firefly (Photinus pyralis)

Firefly Luciferase,荧光素酶抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.