PCDHA1,原钙粘附蛋白α1抗体

产品编号HZ-11115R
英文名称PCDHA1
中文名称原钙粘附蛋白α1抗体
别 名KIAA0345 like 13; PCDH alpha1; PCDHA 1; Protocadherin alpha 1; PCDA1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞粘附分子 细胞骨架 细胞外基质 细胞膜蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog,
PCDHA1,原钙粘附蛋白α1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量100kDa
细胞定位细胞膜 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PCDHA1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PCDHA1,原钙粘附蛋白α1抗体PubMedPubMed
产品介绍background:
Protocadherins are a large family of cadherin-like cell adhesion proteins that are involved in the establishment and maintenance of neuronal connections in the brain. There are three protocadherin gene clusters, designated alpha, beta and gamma, all of which contain multiple tandemly arranged genes. PCDHA1 (protocadherin alpha 1), also known as PCDH-ALPHA1, is a 950 amino acid, single-pass type I membrane protein that contains six cadherin domains. Functioning as a potential calcium-dependent cell-adhesion protein, PCDHA1 has been suspected to play a role in the creation and maintenance of neuronal connections. Three isoforms of PCDHA1 exists due to alternative splicing events.

Function:
PCDHA1 (Protocadherin alpha 1) is a member of the protocadherin family, a subgroup of the cadherin superfamily. Protocadherins are neural cadherin-like cell adhesion proteins that are integral membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific neuronal connections in the brain. PCDHA1 is one of fifteen tandemly arranged genes within the alpha cluster of protocadherin genes on 5q31.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Similarity:
Contains 6 cadherin domains.

Gene ID:
56147

Database links:
Entrez Gene: 56147 Human
Omim: 606307 Human
SwissProt: Q9Y5I3 Human

PCDHA1,原钙粘附蛋白α1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCDHA1) Polyclonal Antibody, Unconjugated (HZ-11115R) at 1:5000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.