Phospho-ATP1A1,***酸化钠钾ATP酶蛋白a1抗体

产品编号HZ-3035R
英文名称Phospho-ATP1A1 (Ser16)
中文名称***酸化钠钾ATP酶蛋白a1抗体
别 名 ATP1A1; Na,K-ATPase alpha-1; Atpa-1; ATPase Na+/K+ transporting alpha 1 polypeptide; BC010319; EC 3.6.3.9; MGC3285; MGC38419; MGC51750; Na K ATPase alpha A catalytic polypeptide; Na K ATPase catalytic subunit alpha A protein; Na(+)/K(+) ATPase 1; Na(+)/K(+) ATPase alpha-1 subunit; Na+/K+ ATPase alpha 1 subunit; Na+/K+ ATPase 1; Nkaa1b; Sodium potassium ATPase alpha 1 polypeptide; Sodium pump 1; Sodium pump subunit alpha-1; Sodium/potassium transporting ATPase alpha 1 chain; Sodium/potassium-transporting ATPase subunit alpha-1.
规格价格0.1ml
说 明 书0.1ml
Phospho-ATP1A1,***酸化钠钾ATP酶蛋白a1抗体产品类型***酸化抗体
研究领域通道蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Guinea Pig,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量110kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ATP1A1 around the phosphorylation site of Ser16
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Phospho-ATP1A1,***酸化钠钾ATP酶蛋白a1抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+-ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May2009].

Function:
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Subunit:
Interacts with SIK1. Composed of three subunits: alpha (catalytic), beta and gamma. Binds the HLA class II histocompatibility antigen, DR1.

Subcellular Location:
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
Phosphorylation on Tyr-10 modulates pumping activity. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity.

Phospho-ATP1A1,***酸化钠钾ATP酶蛋白a1抗体Similarity:
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Gene ID:
476

Database links:
Entrez Gene: 476 Human
Entrez Gene: 11928 Mouse
Entrez Gene: 24211 Rat
Omim: 182310 Human
SwissProt: P05023 Human
SwissProt: Q8VDN2 Mouse
SwissProt: P06685 Rat
Unigene: 371889 Human
Unigene: 280103 Mouse
Unigene: 217534 Rat
Unigene: 2992 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

通道蛋白（Channel Protein）
钠钾ATP酶是位于细胞膜上的一种糖蛋白,与ATP 的分解和细胞内外钠、钾离子的转运密切相关,哺乳动物各种组织细胞的钠钾ATP 酶的免疫学特性基本相同。

产品图片

Tissue/cell: human bladder carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Phospho-ATP1A1(Ser16) Polyclonal Antibody, Unconjugated(bs-3035R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining