PA1-330 Neutralizing Peptide CDFSYNREDFAKA
英文名称： PA1-330 Neutralizing Peptide CDFSYNREDFAKA
型号:null    抗体货号： PEP114
价格:请致电：010-57128832,18610462672
品牌: ******    产品商标: pierce

icken, Dog, Pig, Cow, Horse, Sheep,
APXL,APXL蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量176kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human APXL
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
APXL,APXL蛋白抗体PubMedPubMed
产品介绍background:
APXL is a 1,616 amino acid protein that localizes to a variety of locations within the cell, including the cytoplasm, the cytoskeleton, the cell junction and the apical cell membrane. Containing one ASD1 domain, one ASD2 domain and one PDZ domain, APXL interacts with F-Actin and is thought to mediate endothelial cell morphology during cell spreading, possibly regulating melanosome biogenesis and inducing γ Tubulin redistribution. APXL is expressed in kidney, brain, lung, pancreas and placenta and is overexpressed in melanomas, suggesting a role in tumor transformation and metastasis. The gene encoding APXL maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.

Function:
May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.

Subunit:
Interacts with F-actin (By similarity).

Subcellular Location:
Apical cell membrane (By similarity). Cell junction, tight junction (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Associates with cortical F-actin (By similarity).

Tissue Specificity:
Abundant in retina and melanoma; also in brain, placenta, lung, kidney and pancreas.

Similarity:
Belongs to the shroom family.
Contains 1 ASD1 domain.
Contains 1 ASD2 domain.
Contains 1 PDZ (DHR) domain.

APXL,APXL蛋白抗体Gene ID:
357

Database links:
Entrez Gene: 357 Human
Entrez Gene: 110380 Mouse
Entrez Gene: 317435 Rat
Omim: 300103 Human
SwissProt: Q13796 Human
SwissProt: A2ALU4 Mouse
SwissProt: Q7TP36 Rat
Unigene: 567236 Human
Unigene: 40796 Mouse
Unigene: 16033 Rat


Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 

Purification
 The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Specificity
 Phospho-CRMP-2 (S522) Polyclonal Antibody detects endogenous levels of CRMP-2 protein only when phosphorylated at S522.
Formulation
 Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
 1 mg/ml
Storage / Stability
 -20°C/1 year
Human Gene ID
 1808
Human Swiss-Prot No.
 Q16555
Mouse Gene ID
 12934
Mouse Swiss-Prot No.
 O08553
Rat Gene ID
 25416
Rat Swiss-Prot No.
 P47942
Reactivity
 Human,Mouse,Rat
Applications
 WB,ELISA
Signal Pathway
 Regulation of Microtubule Dynamics
Dilution
 Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Predicted band (Da)
 62294
Observed band (KDa)
 62
  CRMP-2 (phospho Ser522) Polyclonal Antibody Images
Western Blot analysis of Hela cells using Phospho-CRMP-2 (S522) Polyclonal Antibody diluted at 1：2000