NSF,N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体

产品编号HZ-11255R
英文名称NSF
中文名称N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体
别 名N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor; N ethylmaleimide sensitive factor like protein; N ethylmaleimide sensitive fusion protein; N ethylmaleimide sensitive fusion protein; N-ethylmaleimide-sensitive fusion protein; NEM sensitive fusion protein; NEM sensitive fusion protein; NEM-sensitive fusion protein; NSF; NSF_HUMAN; SKD 2; SKD2; SKD2; Vesicle fusing ATPase; Vesicle fusing ATPase; Vesicle-fusing ATPase; Vesicular fusion protein NSF; Vesicular fusion protein NSF; Vesicular-fusion protein NSF.
说 明 书0.1ml 0.2ml
研究领域肿瘤 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep,
NSF,N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量83kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NSF
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NSF,N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体PubMedPubMed
产品介绍background:
Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).

Function:
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.

Subunit:
Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity).

Subcellular Location:
Cytoplasm.

Post-translational modifications:
Phosphorylation at Ser-569 interferes with homohexamerization (By similarity).

Similarity:
Belongs to the AAA ATPase family.

NSF,N-乙基顺丁烯二酰亚胺敏感融合蛋白抗体Database links:
Entrez Gene: 4905 Human
Entrez Gene: 18195 Mouse
Entrez Gene: 60355 Rat
Omim: 601633 Human
SwissProt: P46459 Human
SwissProt: P46460 Mouse
SwissProt: Q9QUL6 Rat
Unigene: 431279 Human
Unigene: 260117 Mouse
Unigene: 13345 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.