E2EPF,泛素交联酶抗体

产品编号HZ-2305R
英文名称E2EPF
中文名称泛素交联酶抗体
别 名E2 EPF; E2EPF; EPF5; UBE2S; Ubiquitin carrier protein S; Ubiquitin conjugating enzyme E2 24 kD; Ubiquitin conjugating enzyme E2S; Ubiquitin protein ligase S; AA409170; MGC101982; UBE2S_HUMAN.
说 明 书0.1ml 0.2ml
研究领域免疫学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
E2EPF,泛素交联酶抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量24kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human E2EPF
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E2EPF,泛素交联酶抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq, Jul 2008]

Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.

Subunit:
Component of the APC/C complex. Interacts with CDC20, FZR1/CDH1 and VHL.

Post-translational modifications:
Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.

Similarity:
Belongs to the ubiquitin-conjugating enzyme family.

Gene ID:
27338

E2EPF,泛素交联酶抗体Database links:
Entrez Gene: 617703 Cow
Entrez Gene: 27338 Human
Entrez Gene: 77891 Mouse
Entrez Gene: 292588 Rat
GenBank: NP_055316.2 Human
Omim: 610309 Human
SwissProt: Q1RML1 Cow
SwissProt: Q16763 Human
SwissProt: Q921J4 Mouse
SwissProt: B5DFI8 Rat
Unigene: 396393 Human
Unigene: 389787 Mouse
Unigene: 120398 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.