COL6A5,6型胶原蛋白α5抗体

产品编号HZ-11047R
英文名称COL6A5
中文名称6型胶原蛋白α5抗体
别 名Collagen VI alpha 5; CO6A5_HUMAN; COL29A1; Col6a5; Collagen alpha-1(XXIX) chain; Collagen alpha-5(VI) chain; Collagen, type VI, alpha 5; Collagen, type XXIX, alpha-1; Gm7455; von Willebrand factor A domain-containing protein 4; VWA4.
说 明 书0.1ml 0.2ml
研究领域发育生物学 神经生物学 信号转导 细胞粘附分子 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,
COL6A5,6型胶原蛋白α5抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量288kDa
细胞定位细胞外基质 分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human COL6A5/Collagen VI alpha 5
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
COL6A5,6型胶原蛋白α5抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is a collagen that contains N- and C-terminal von Willebrand factor A-like domains. The encoded protein may interact with the alpha 1 and alpha 2 chains of collagen VI to form the complete collagen VI trimer. Sequence polymorphisms in this gene have been linked to atopic dermatitis (eczema). Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene.

Function:
Collagen VI acts as a cell-binding protein.

Subunit:
Trimers composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI)

Subcellular Location:
Secreted, extracellular space, extracellular matrix. Note=Deposed in the extracellular matrix of skeletal muscle.

Tissue Specificity:
Expressed in skin, followed by lung, small intestine, colon and testis. In skin, it is expressed in the epidermis with strongest staining in suprabasal viable layers. In ATOD patients, it is absent in the most differentiated upper spinous and granular layers (at protein level).

Post-translational modifications:
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains

DISEASE:
Note=Patients affected by atopic dermatitis display an abnormal distribution of COL29A1 mRNA and protein in skin suggesting that COL29A1 may be involved in the pathogenesis of the disease.

COL6A5,6型胶原蛋白α5抗体Similarity:
Belongs to the type VI collagen family.
Contains 10 VWFA domains.

Database links:
Entrez Gene: 256076 Human
Omim: 611916 Human
SwissProt: A8TX70 Human
Unigene: 205403 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-COL6A5/Collagen VI alpha 5 Polyclonal Antibody, Unconjugated(HZ-11047R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining