DENND1B,DENND1B蛋白抗体

产品编号HZ-8254R
英文名称DENND1B
中文名称DENND1B蛋白抗体
别 名C1ORF18; C1orf218; Chromosome 1 open reading frame 218; Connecdenn 2; DEN1B_HUMAN; DENN domain containing protein 1B; DENN domain-containing protein 1B; DENN MADD domain containing 1B; DENND1B; DKFZp547O0715; FAM31B; Family with sequence similarity 31 member B; FLJ20054; FLJ21179; FLJ33888; MGC103810; MGC27044; Protein FAM31B
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
DENND1B,DENND1B蛋白抗体交叉反应Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量48kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human DENND1B
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DENND1B,DENND1B蛋白抗体PubMedPubMed
产品介绍background:
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactived RAB35, then activates RAB35 for its subsequent function at endosomes.
Tissue specificity:Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T cells as compared with naive T cells.

Function:
Guanine nucleotide exchange factor (GEF) for RAB35. May function as a clathrin-associated sorting protein for RAB35, whereby during clathrin-mediated endocytosis, binds the AP-2 complex and inactivates RAB35, then activates RAB35 for its subsequent function at endosomes.

Subunit:
Interacts with RAB35 in the absence of nucleotide, but not in the presence of GTP. Interacts with clathrin heavy chain/CLTC and with AP2A2. Isoform 5 interacts with AP2B1.

Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle, clathrin-coated vesicle.

DENND1B,DENND1B蛋白抗体Tissue Specificity:
Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells.

Post-translational modifications:
Isoform 5 is phosphorylated on Tyr-475, Tyr-484, Ser-514 and Ser-652.

Similarity:
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.

DENND1B,DENND1B蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.