Anti-active Arl1 Mouse Monoclonal Antibody
英文名称： Anti-active Arl1 Mouse Monoclonal Antibody
型号:null    抗体货号： 26924
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Active Arl1-GTP [26924]
[30 µL]		  

Catalog Number:  26924
Gene Symbol:  Arl1
Description:   Anti-active Arl1 Mouse Monoclonal Antibody
Background:   Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal.
Immunogen:   Recombinant Arl1 protein (active form)
Applications:   IP, IHC, IF
Recommended Dilutions:   
1 µg for 1~2 mg total cellular proteins for IP
1:50~100 dilution for IHC, IF
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Arl1 antibody recognizes active Arl1-GTP from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  

 

Arl1 Activation Assay. Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti-active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti- Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel.