Anti-Active Gα13-GTP Mouse Monoclonal Antibody
英文名称： Anti-Active Gα13-GTP Mouse Monoclonal Antibody
型号:null    抗体货号： 26902
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Active Gα13-GTP [26902]
[30 µL]		  

Catalog Number:  26902
Gene Symbol:  Gna13
Description:   Anti-Active Gα13-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Immunogen:   Recombinant full length active Gα13 protein
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004).
Immunohistochemistry:  
Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.
 

 

Publications:
1.  Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization
    J Biol Chem. 2011 Sep 2;286(35):31055-61
2.  Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2
    Br J Pharmacol. 2011 Feb;162(3):733-48
3.  Gα12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation
    Carcinogenesis (2010)
4.  Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency
    J Biol Chem. 2009 Oct 2;284(40):27409-15