美国产品商标: neweastbioBrand,null 抗体货号： 26901Item#, Anti-Active Gα i Mouse Monoclonal Antibody<br>英文名称： Anti-Active Gα i Mouse Monoclonal Antibody

Active Gαi-GTP [26901]
[30 µL]

Catalog Number: 26901

Gene Symbol: Gnai

Description: Anti-Active Gα _i Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα _i represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα _i proteins are being explored.

Immunogen: Recombinant full length protein of active Gα _i1

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG1

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg ²⁺and Ca ²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Gα _i antibody recognizes active Gα _i1, Gα _i2, and Gα _i3 of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Gα_i activation assay.
A. CHO cells were transfected with wild-type Gα _i1 (lanes 1 and 2) or constitutively active Gα _i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα _i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα _i was immunoblotted with an anti-Gα _i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα _i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα _i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα _i was immunoblotted with an anti-Gα _i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.

Publications:

1. Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells
Biochem J. 2013 Feb 15;450(1):209-19

2. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61

3. CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma
Cancer Res. 2012 Jan 1;72(1):122-32

4. Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts
Endocrinology. 2012 Oct;153(10):4918-28

5. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
Cell Signal. 2011 Mar;23(3):550-4

6. Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis
Biochim Biophys Acta. 2011 Mar;1813(3):466-72

7. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I
Steroids Volume 77, Issue 5, April 2012, Pages 424–432

8. Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
J Biol Chem. 2010 Aug 27;285(35):26976-86

9. Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7
Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32

10. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin
Mol Biol Cell. 2014 Nov 5;25(22):3654-71

11. Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
Mol Cell Biol. 2014 Nov 15;34(22):4186-99

12. Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain
J Biol Chem. 2014 May 2;289(18):12748-58