Anti-Active Gαo Mouse Monoclonal Antibody
英文名称: Anti-Active Gαo Mouse Monoclonal Antibody
型号:null    抗体货号: 26907
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

Active Gαo-GTP [26907]
[30 µL]
 

Catalog Number:  26907
Gene Symbol:  Gnao
Description:   Anti-Active Gαo Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαo
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
o activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαo polyclonal antibody (Cat. No. 21015).