Catalog Number:  26908

Gene Symbol:  Gnaz

Description:   Anti-Active Gαz Mouse Monoclonal Antibody

Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαz represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαz proteins are being explored.

Immunogen:   Recombinant full length protein of active Gαz

Applications:    IP, IHC

Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG

Purity:   Purified from ascites

Storage buffer:

Preservative:  no

Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active Gαz antibody recognizes active Gαz of vertebrates.

Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Gαz activation assay. Purified Gαz proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαz monoclonal antibody (Cat. No. 26908). Immunoblot was with an anti-Gαz