Background: Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen: Recombinant full length protein of active Gαo
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG1
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Gαo activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαo polyclonal antibody (Cat. No. 21015).