美国产品商标: neweastbioBrand,null 抗体货号： 26906Item#, Anti-Active Gαs-GTP Mouse Monoclonal Antibody<br>英文名称： Anti-Active Gαs-GTP Mouse Monoclonal Antibody

Active Gαs-GTP [26906]
[30 µL]

Catalog Number: 26906

Gene Symbol: Gnas

Description: Anti-Active Gα_s-GTP Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are critical cellular signal transducers. Gα_s represents one sub-family of G proteins that could mediate the stimulation of adenylyl cyclases.

Immunogen: Recombinant full length protein of active Gα_s

Applications: IP, IHC

Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP;
1:100 dilution for IHC

Concentration: 1 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Anti-active Gα_s antibody recognizes active Gα_s of vertebrates

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Gα_s activation assay. Purified Gα_s proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gα_s monoclonal antibody (Cat. No. 26906). Immunoblot was with an anti- Gα_s monoclonal antibody (Cat. No. 26006).

Publications:

1. Glucocorticoid acts on a putative G protein-coupled receptor to rapidly regulate the activity of NMDA receptors in hippocampal neurons
Am J Physiol Endocrinol Metab. 2012 Apr 1;302(7):E747-58

2. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61

3. Estrogenic action on arterial smooth muscle: permissive for maintenance of CRHR2 expression
Endocrinology. 2012 Apr;153(4):1915-24

4. Corticotropin-releasing hormone stimulates mitotic kinesin-like protein 1 expression via a PLC/PKC-dependent signaling pathway in hippocampal neurons
Mol Cell Endocrinol. 2012 Oct 15;362(1-2):157-64

5. Rapid actions of xenoestrogens disrupt normal estrogenic signaling
Steroids Volume 81, March 2014, Pages 36–42

6. Neuronal NF1/RAS regulation of cyclic AMP requires atypical PKC activation
Hum. Mol. Genet. (2014)