Catalog Number:  26919

Gene Symbol:  RAB11

Description:   Anti-Active Rab11 Mouse Monoclonal Antibody

Background:   Small GTPases of the Rab-family are crucial elements of the machinery that controls membrane traffic. Rab11 has been shown to control traffic through the recycling endosome.

Immunogen:   Recombinant full length protein of active Rab11

Applications:   IP, IHC

Recommended Dilutions:   IP: 1 μg for 1~2 mg total cellular proteins IHC: 1:50~100 dilution

Concentration:   1 mg/ml

Host:   mouse

Format:   Liquid

Clonality:   Monoclonal

Isotype:   IgG2b

Purity:    Purified from ascites

Storage buffer:

Preservative:  no

Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity:  Anti-active Rab11 antibody recognizes active Rab11 from vertebrates.

Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

Purified full-length Rab11 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-active Rab11 monoclonal antibody (Cat. No. 26919). Immunoblot was with an anti-Rab5 polyclonal antibody (Cat. No. 21157).

Publications:

1.  VASP promotes TGF-β activation of hepatic stellate cells by regulating Rab11 dependent plasma membrane targeting of TGF-β receptors     Hepatology Volume 61, Issue 1, pages 361–374, January 2015

2.  Rab5 Activity Regulates GLUT4 Sorting into ***-Responsive and Non-***-Responsive Endosomal Compartments: A Potential Mechanism for Development of *** Resistance     Endocrinology. 2014 Sep;155(9):3315-28