Apaf1 Interacting Protein,凋亡蛋白活性因子1相互作用蛋白抗体

产品编号HZ-6792R
英文名称Apaf1 Interacting Protein
中文名称凋亡蛋白活性因子1相互作用蛋白抗体
别 名MTNB_HUMAN; Apaf1 Interacting Protein; APIP2; CG129; CGI 29; MMRP19; MTRu 1 P dehydratase; Probable methylthioribulose 1 phosphate dehydratase.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
Apaf1 Interacting Protein,凋亡蛋白活性因子1相互作用蛋白抗体交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human APIP (178-225aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Apaf1 Interacting Protein,凋亡蛋白活性因子1相互作用蛋白抗体PubMedPubMed
产品介绍background:
The mammalian homologues of the key cell death gene CED 4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase 9 (Apaf3), which leads to caspase 9 activation. Activated caspase 9 in turn cleaves and activates caspase 3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. A new Apaf1 Interacting Protein (APIP) also known as CG129 and MMRP19, has been identified as a negative regulator of ischemic injury. APIP competes with Caspase 9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.

Function:
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Has an anti-apoptotic function and prevents muscle ischemic damage. Inhibits the cytochrome c-dependent and APAF1-mediated cell death.

Subunit:
Interacts with APAF1.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Ubiquitously expressed with high expression in skeletal muscle, heart and kidney.

Similarity:
Belongs to the aldolase class II family. MtnB subfamily.

Gene ID:
51074

Apaf1 Interacting Protein,凋亡蛋白活性因子1相互作用蛋白抗体Database links:
Entrez Gene: 508345 Cow
Entrez Gene: 475943 Dog
Entrez Gene: 32334 Fruit fly (Drosophila melanogaster)
Entrez Gene: 100732536 Guinea pig
Entrez Gene: 51074 Human
Entrez Gene: 56369 Mouse
Entrez Gene: 100525733 Pig
Entrez Gene: 295961 Rat
Entrez Gene: 447941 Zebrafish
Omim: 612491 Human
SwissProt: Q0VCJ2 Cow
SwissProt: Q9VY93 Fruit fly (Drosophila melanogaster)
SwissProt: Q96GX9 Human
SwissProt: Q8BP46 Mouse
SwissProt: Q9WVQ5 Mouse
SwissProt: Q66I75 Zebrafish
Unigene: 5343 Fruit fly (Drosophila melanogaster)
Unigene: 447794 Human
Unigene: 24772 Mouse
Unigene: 15452 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.