TCAB1,端粒酶卡哈尔体蛋白抗体

产品编号HZ-8163R
英文名称TCAB1
中文名称端粒酶卡哈尔体蛋白抗体
别 名FLJ10385; TCAB1; telomerase Cajal Body protein 1; WD repeat containing protein 79; WRAP53; WDR79/TCAB1; WAP53_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Horse, Rabbit,
TCAB1,端粒酶卡哈尔体蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量60kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human WDR79/TCAB1（304-347aa）
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
TCAB1,端粒酶卡哈尔体蛋白抗体PubMedPubMed
产品介绍background:
WDR79 contains six WD (tryptophan-aspartate) repeat domains found in a number of proteins that function as adaptor molecules in signal transduction and cytoskeletal organization. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. The function of the WDR79 protein has not been characterized, however significant and consistent single nucleotide polymorphisms in the WDR79 gene have been found to be associated with ER negative breast cancer.

Function:
Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes. In the telomerase holoenzyme complex, it controls telomerase localization to Cajal body. Required for delivery of TERC to telomeres during S phase and for telomerase activity. Binds small Cajal body RNAs (scaRNAs). The mRNA encoding this protein plays a critical role in the regulation of p53 expression at the post-transcriptional level; it is involved both in maintaining basal p53 mRNA levels and in p53 induction upon DNA damage.

TCAB1,端粒酶卡哈尔体蛋白抗体Subunit:
Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC).

Subcellular Location:
Nucleus, Cajal body. Cytoplasm.

Tissue Specificity:
Expressed in all tissues and cell lines examined.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

DISEASE:
Defects in WRAP53 are the cause of dyskeratosis congenital autosomal recessive type 3 (DKCB3) [MIM:613988]. A rare multisystem disorder caused by defective telomere maintenance. It is characterized by progressive bone marrow failure, and the clinical triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Common but variable features include premature graying, aplastic anemia, low platelets, osteoporosis, pulmonary fibrosis, and liver fibrosis among others. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.

Similarity:
Contains 6 WD repeats

Database links:

UniProtKB/Swiss-Prot: Q9BUR4.1

TCAB1,端粒酶卡哈尔体蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

TCAB1是端粒酶的一个真正组成部分。但它对酶的活性来说并不是必需的，它只是给称为卡哈尔体（Cajalbodies）的细胞核中的处理和保持区域补充端粒酶复合物。卡哈尔体将对各种使用RNA小分子来引领其活性的蛋白进行修饰，譬如，端粒酶使用RNA分子作为嵌在染色体末端的DNA链的模板。在适当的时候，TCAB1将端粒酶复合物运送到新复制染色体的等待端。 研究人员认为，TCAB1蛋白可能是一种负责将各种分子运往其目的地的普通生物运输器。