Cyclin C,周期素C抗体

产品编号HZ-0282R
英文名称Cyclin C
中文名称周期素C抗体
别 名CCNC; Cyclin-C; CycC; Cyclin C; hSRB11; SRB11 homolog; OTTHUMP00000016897; CCNC_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 染色质和核信号 细胞周期蛋白 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
Cyclin C,周期素C抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Cyclin C C-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Cyclin C,周期素C抗体PubMedPubMed
产品介绍background:
Cyclin C was originally identified by a genetic screen for human and Drosophila cDNAs that complement a triple knock out of the CLN genes in Saccharomyces cerevisiae. Cyclin C is a nuclear protein present in a multiprotein complex. It interacts both in vitro and in vivo with cdk 8, a novel protein kinase of the cdk family. It is suggested that cyclin C and cdk 8 control RNA polymerase II function. Human cdk 8 cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth regulatory signals.

Function:
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. [SUBUNIT] Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. The cylin/CDK pair formed by CCNC/CDK8 also associates with the large subunit of RNA polymerase II.

Cyclin C,周期素C抗体Subcellular Location:
Nucleus (Probable).

Tissue Specificity:
Highest levels in pancreas. High levels in heart, liver, skeletal muscle and kidney. Low levels in brain.

Similarity:
Belongs to the cyclin family. Cyclin C subfamily.
Contains 1 cyclin N-terminal domain.

Gene ID:
892

Database links:
Entrez Gene: 892 Human
Entrez Gene: 51813 Mouse
Entrez Gene: 114839 Rat
Omim: 123838 Human
SwissProt: P24863 Human
SwissProt: Q62447 Mouse
SwissProt: P39947 Rat
Unigene: 430646 Human
Unigene: 278584 Mouse
Unigene: 106758 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

周期素 C属于高度保守的周期素家族。 不同的周期素出现在不同的细胞增殖周期。 能结合和激活CDK激酶。
周期素 C(Cyclin C)主要出现在G1期（第一间隙期（或生长期）（G1）：是在有丝分裂（M）和开始DNA合成（S）期之间的阶段；

产品图片

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Cyclin C Polyclonal Antibody, Unconjugated(HZ-0282R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining