phospho-IKKi(Ser172),***酸化核因子NFκB抑制蛋白激酶i抗体

产品编号HZ-8583R
英文名称phospho-IKKi(Ser172)
中文名称***酸化核因子NFκB抑制蛋白激酶i抗体
别 名IKKi/IKKe (phospho Ser172); p-IKKi(Ser172); p-IKKe(Ser172); IKKi/IKKe; I kappa B kinase epsilon; IkBKE; IKBKE protein; IKK E; IKK i; IKK-i; IKK related kinase epsilon; IKKE; IKK-E; IKKepsilon; IKKI; Inducible I kappa B kinase; inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of nuclear factor kappa B kinase subunit epsilon; KIAA0151; MGC125294; MGC125295; MGC125297; IKKE_HUMAN; I-kappa-B kinase epsilon; IKK-epsilon; Inducible I kappa-B kinase.
说 明 书0.1ml
产品类型***酸化抗体
研究领域肿瘤 细胞生物 细胞凋亡 细胞周期蛋白 激酶和***酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
phospho-IKKi(Ser172),***酸化核因子NFκB抑制蛋白激酶i抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量80kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human IKKi/IKKE around the phosphorylation site of Ser172 [FV(p-S)VY]
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-IKKi(Ser172),***酸化核因子NFκB抑制蛋白激酶i抗体PubMedPubMed
产品介绍background:
IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009].

Function:
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

Subunit:
May interact with MAVS/IPS1. Interacts with AZI2. Interacts with SIKE1. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between IKBKE and SIKE1. Interacts with TOPORS; induced by DNA damage. Interacts with CYLD.

Subcellular Location:
Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent.

phospho-IKKi(Ser172),***酸化核因子NFκB抑制蛋白激酶i抗体Tissue Specificity:
Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon.

Post-translational modifications:
Autophosphorylated and phosphorylated by IKBKB/IKKB.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.

Gene ID:
9641

Database links:
Entrez Gene: 9641 Human
Omim: 605048 Human
SwissProt: Q14164 Human
Unigene: 321045 Human

phospho-IKKi(Ser172),***酸化核因子NFκB抑制蛋白激酶i抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-IKKi (Ser172) Polyclonal Antibody, Unconjugated(HZ-8583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-IKKi(Ser172) Polyclonal Antibody, Unconjugated(HZ-8583R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining