BSX,脑特异性同源蛋白BSX抗体

产品编号HZ-12905R
英文名称BSX
中文名称脑特异性同源蛋白BSX抗体
别 名Brain specific homeobox; Brain specific homeobox protein homolog; BSH; BSX1; BSH_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 转录调节因子 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
BSX,脑特异性同源蛋白BSX抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量26kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human BSX
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
BSX,脑特异性同源蛋白BSX抗体PubMedPubMed
产品介绍background:
BSX(Brain-specific homeobox protein homolog) may play a role in the determination and function of cell types in the brain. In brain, it is restricted to a few specific developing brain structures such as pineal gland, telencephalic septum, hypothalamic pre-mammillary body and arcuate nucleus. Bsx might be considered an important molecular marker for early embryonic stages of epiphysis development. It belongs to the distal-less homeobox family and contains 1 homeobox DNA-binding domain.

Function:
DNA binding protein that function as transcriptionalactivator. Is essentiel for normal postnatal growth and nursing. Isan essential factor for neuronal neuropeptide Y and agouti-relatedpeptide function and locomotory behavior in the control of energybalance (By similarity).

Subcellular Location:
Nuclear

Similarity:
Belongs to the distal-less homeobox family.
Contains 1 homeobox DNA-binding domain.

BSX,脑特异性同源蛋白BSX抗体Database links:
UniProtKB/Swiss-Prot: Q3C1V8.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Blank control: RSC96(blue).  Primary Antibody:Rabbit Anti-BSX antibody(HZ-12905R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.The cells were washed twice with phosphate-buffered saline (PBS).The cells were then incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (HZ-12905R, 1μg /1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.