- Product Description:
- Goat polyclonal antibody raised against synthetic peptide of IDH1.
- Immunogen:
- A synthetic peptide corresponding to internal region of Saccharomyces cerevisiae (S288c) IDH1.
- Sequence:
- C-EPGSRHVGLDIKGQN
- Theoretical MW (kDa):
- 39.3
- Purification:
- Ammonium sulfate precipitation
- Storage Buffer:
- In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Recommend Usage:
- ELISA (1:1600)
Western Blot (0.3-1 ug/mL) The optimal working dilution should be determined by the end user. - Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
- Western Blot (Cell lysate)
 - IDH1 polyclonal antibody (Cat # PAB19472)(0.5 ug/mL) staining of S. cerevisiae lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
-
- Other Designations:
- Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
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