英文名称： Streptavidin polyclonal antibody
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against native streptavidin.
- Immunogen:
- Native purified streptavidin from Streptomyces avidinii.
- Host:
- Rabbit
- Form:
- Liquid
- Purification:
- Ion exchange chromatography purification
- Storage Buffer:
- In 20 mM K3PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:200000)
Immunohistochemistry (1:1500-1:5000)
Western Blot (?:3000-1:12000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- Western Blot (Recombinant protein)
- Streptavidin and Biotin conjugated Rabbit anti-Trypsin inhibitor antibody were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of Streptavidin polyclonal antibody (Cat # PAB24945). Detection shown was using Donkey anti Rabbit (Dylight 649) (1:10, 000 1.5 hr RT in blocking buffer) and imaged on the BioRad VersaDoc System.