英文名称： Beta-Galactosidase polyclonal antibody
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against native Galactosidase-beta.
- Immunogen:
- Native purified Escherichia coli Galactosidase-beta.
- Host:
- Rabbit
- Reactivity:
- Escherichia coli, Human, Mouse
- Form:
- Liquid
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Native Purified Protein.
- Recommend Usage:
- ELISA (1:10000)
Western Blot (0.5-1.0 ug/mL)
Immunocytochemistry (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitation (5ug/300uL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- High-Level Expression of Lactobacillus beta-Galactosidases in Lactococcus lactis Using the Food-Grade, Nisin-Controlled Expression System NICE.
Maischberger T, Mierau I, Peterbauer CK, Hugenholtz J, Haltrich D.J Agric Food Chem. 2010 Jan 21.
- 2.
- Bioinformatics Characterization of Potential New Beta-Glucuronidase from Streptococcus equi subsp. zooepidemicus.
Krahulec J, Szemes T, Krahulcova J.Mol Biotechnol. 2010 Jan 14.
- 3.
- Synthesis and glycosidase inhibitory activity of noeurostegine-a new and potent inhibitor of beta-glucoside hydrolases.
Rasmussen TS, Jensen HH.Org Biomol Chem. 2010 Jan 21;8(2):433-41. Epub 2009 Nov 23.
- Applications
- Western Blot (Recombinant protein)
- Western blotting using Beta-Galactosidase polyclonal antibody (Cat # PAB9921) shows a band at ~117 kDa (Lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of Beta-Galactosidase present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.