英文名称： Aspergillus Terrus Blasticidin S Deaminase polyclonal antibody
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Aspergillus Terrus Blasticidin S Deaminase.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of Aspergillus Terrus Blasticidin S Deaminase.
- Host:
- Rabbit
- Reactivity:
- Fungi
- Form:
- Liquid
- Storage Buffer:
- In PBS (50% glycerol, 1 mg/mL BSA, 0.05% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:4000)
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Blasticidin S deaminase gene from Aspergillus terreus (BSD): a new drug resistance gene for transfection of mammalian cells.
Kimura M, Takatsuki A, Yamaguchi I.Biochim Biophys Acta. 1994 Nov 22;1219(3):653-9.
- 2.
- Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells.
Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F.Exp Cell Res. 1991 Dec;197(2):229-33.
- Applications
- Western Blot (Transfected lysate)
- Western blot of 293 cells mock transfected (lane 1) or transiently transfected with pLenti6/TR lentiviral vector (lanes 2 & 3). Blots were probed with Blasticidin S Deaminase polyclonal antibody (Cat # PAB7931) (lanes 1-3). Molecular weight (MW) standards show that Blasticidin S Deaminase is expressed as a 13 KDa band in only the transfected cells.
- Immunofluorescence
- Immunocytochemical labeling of Blasticidin S Deaminase in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were labeled with Blasticidin S Deaminase polyclonal antibody (Cat # PAB7931) and detected using appropriate secondary antibody conjugated to Texas Red. (Images provided by Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center).