SARP1,分泌细胞凋亡相关蛋白1抗体

产品编号HZ-9478R
英文名称SARP1
中文名称分泌细胞凋亡相关蛋白1抗体
别 名Frizzled related protein 2; FRP 2; FRP-2; FRP2; SARP 1; SARP-1; SARP1; SDF 5; SDF5; Secreted apoptosis related protein 1; Secreted apoptosis-related protein 1; Secreted frizzled related protein 2; Secreted frizzled-related protein 2; sFRP 2; sFRP-2; sFRP2; Sfrp2 protein; SFRP2_HUMAN.
说 明 书0.1ml 0.2ml
研究领域心血管 信号转导 干细胞 细胞凋亡 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, Sheep,
SARP1,分泌细胞凋亡相关蛋白1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SFRP2/SARP1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SARP1,分泌细胞凋亡相关蛋白1抗体PubMedPubMed
产品介绍background:
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Function:
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Subcellular Location:
Secreted.

Tissue Specificity:
Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Similarity:
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain.

Gene ID:
6423

Database links:
Entrez Gene: 6423 Human
Entrez Gene: 20319 Mouse
Entrez Gene: 310552 Rat
Omim: 604157 Human
SwissProt: Q96HF1 Human
SwissProt: P97299 Mouse
Unigene: 481022 Human
Unigene: 19155 Mouse
Unigene: 102416 Rat

SARP1,分泌细胞凋亡相关蛋白1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Western blot analysis of extracts from Hel using SARP1 antibody. (Primary dilution:1:300) Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SARP1 Polyclonal Antibody, Unconjugated(HZ-9478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SARP1 Polyclonal Antibody, Unconjugated(HZ-9478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human rectal cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SARP1 Polyclonal Antibody, Unconjugated(HZ-9478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SARP1 Polyclonal Antibody, Unconjugated(HZ-9478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining