IGSF12,免疫球蛋白超家族成员12抗体

产品编号HZ-9202R
英文名称IGSF12
中文名称免疫球蛋白超家族成员12抗体
别 名IGSF12; CD300 antigen-like family member A; CD300a; CD300a molecule; CLM-8; CLM8_HUMAN; CMRF 35 H9; CMRF-35-H9; CMRF35-H; CMRF35-H9; CMRF35-like molecule 8; CMRF35H leukocyte immunoglobulin like receptor; CMRF35H9; IgSF12; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; Leukocyte membrane antigen; Mast cell derived paired immunoglobulin like receptor 1; NK inhibitory receptor; Polymeric immunoglobulin receptor 4.
说 明 书0.1ml 0.2ml
研究领域免疫学 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
IGSF12,免疫球蛋白超家族成员12抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量31kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human IGSF12/CD300
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
IGSF12,免疫球蛋白超家族成员12抗体PubMedPubMed
产品介绍background:
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.

Function:
Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.

Subunit:
Upon tyrosine-phosphorylation, interacts with PTN6/SHP-1 and PTPN11/SHP-2 and INPP5D.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein (Potential).

Tissue Specificity:
Expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes.

Post-translational modifications:
Phosphorylated on tyrosine.

Similarity:
Belongs to the CD300 family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Gene ID:
11314

IGSF12,免疫球蛋白超家族成员12抗体Database links:
Entrez Gene: 11314 Human
Omim: 606790 Human
SwissProt: Q9UGN4 Human
Unigene: 9688 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Western blot analysis of extracts from U937 cell line using IGSF12 antibody. (Primary dilution:1:300) Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-IGSF12 Polyclonal Antibody, Unconjugated(HZ-9202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-IGSF12 Polyclonal Antibody, Unconjugated(HZ-9202R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining