- 样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media - 检测类型Semi-quantitative
- 种属反应性
与反应: Human - 产品概述
ab134004 is for simultaneous detection of 7 Human MMPs and 3 TIMPs. Suitable for all sample types. Targets: MMP-1, MMP-2, MMP-3, MMP-8, MMP-9, MMP-10, MMP-13, TIMP-1, TIMP-2, TIMP-4 Learn more about membrane antibody arrays - 经测试应用Multiplex Protein Detectionmore details
性能 - 存放说明Store at -20°C. Please refer to protocols.
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| 组件 | 1 x 4 Membranes | 1 x 8 Membranes | | 1000X HRP-Conjugated Streptavidin | 1 x 50µl | 1 x 50µl | | 1X Blocking Buffer | 1 x 25ml | 1 x 50ml | | 20X Wash Buffer I | 1 x 10ml | 1 x 20ml | | 20X Wash Buffer II | 1 x 10ml | 1 x 20ml | | 2X Cell Lysis Buffer | 1 x 10ml | 1 x 20ml | | 8-Well Plastic Tray | 1 unit | 1 unit | | Biotin-Conjugated Anti-Cytokines | 2 vials | 4 vials | | Cytokine Antibody Array Membranes | 4 units | 8 units | | Detection Buffer C | 1 x 1.5ml | 1 x 2.5ml | | Detection Buffer D | 1 x 1.5ml | 1 x 2.5ml | - 研究领域
应用Our Abpromise guarantee covers the use of ab134004 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. | 应用 | Abreviews | 说明 | | Multiplex Protein Detection | | Use at an assay dependent concentration. | Human MMP Antibody Array - Membrane (10 Targets) 图像 -
 Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004) Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. -
 Quantification of Human MMP Antibody Array Human serum and plasma from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis. -
 Multiplex Protein Detection - Human MMP Antibody Array - Membrane (10 Targets) (ab134004) 143B cells (Human osteosarcoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. -
 Quantification of Human MMP Antibody Array Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134004. Mean pixel density was quantified using CCD camera software analysis. -
 Dot Blot - Human MMP Antibody Array - Membrane (10 Targets) (ab134004)This image is courtesy of an anonymous customer |