Rac信号传导分子激活检测试剂盒
英文名称: Rac Activation Assay Kit
型号:null    抗体货号: 80501
价格:请致电:010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Rac Activation Assay Kit [80501]
[20 assays]
 

Product Highlight

  • Configuration-specific monoclonal anti-Rac-GTP antibody based
  • This monoclonal antibody specifically recognizes Rac-GTP, but not Rac-GDP
  • Providing shorter experimental time
  • Providing reliable results with consistent reproducibility than other Rac activation assay kits
  • Could be used to monitor the activation of Rac in cells and in tissues by immunohistochemistry

Product Description

    Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive.

    Currently the activation of Rac proteins is assayed with the binding of GTP-bound Rac to the p21-binding domain (PBD) of p21-activated protein kinase (PAK). This method is based on the observation that the active, GTP-bound Rac could bind to the PBD of PAK. However, the reproducibility of this method is poor. This is partially due to the relatively quick hydrolysis of GTP to GDP during the assay procedure, and the low binding affinity of PBD to Rac-GTP.

    These anti-Rac-GTP monoclonal antibody can also be used to monitor the activation of Rac in cells and in tissues by immunohistochemistry.

    NewEast Biosciences Rac Activation Assay Kit provides a simple and fast method to monitor the activation of Rac. Each kit provides sufficient quantities to perform 20 assays.

     

Assay Principle