大鼠抑制素A(INH-A)酶联免疫分析检测试剂盒
英文名称： 电询
型号:null    抗体货号：  YM-YM6835
价格:请致电：010-57128832,18610462672
品牌: 上海    产品商标: 上海延慕

上海延慕ELISA试剂盒供应商！
注：本公司产品仅用于科研实验！

大鼠抑制素A(INH-A)酶联免疫分析检测试剂盒上海延慕主营：ELISA试剂盒、标准品对照品、生化试剂、微生物培养基、生物分子、抗体、动物血清、染色液等等。
产品名：大鼠抑制素A(INH-A)酶联免疫分析检测试剂盒
搜索名：大鼠抑制素A(INH-A)ELISA试剂盒
货号：YM-YM6835
规格：48T/96T

试剂盒组成：

 上海延慕生物有限公司

大鼠抑制素A(INH-A)酶联免疫分析检测试剂盒
ELISA试剂盒性能：
试剂盒性能：
1.样品线性回归与预期浓度相关系数R值为0.990以上。
2.批内与批见应分别小于9%和11%。
性能：
1.准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。
2.灵敏度：最低检测浓度小于10 pg/ml。
3.特异性：不与其它可溶性结构类似物交叉反应。
4.重复性：板内、板间变异系数均小于15%。
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标签：ELISA检测试剂盒  免疫免疫分析试剂盒  免疫免疫试剂盒  ELISA试剂盒免费代测

oire of ligands, from photons to large peptides, activates G protein-coupled receptors (GPCRs) to elicit their physiological functions. Ligand-bound GPCRs, in turn, function as guanine nucleotide exchange factors catalyzing the exchange of GDP bound on the Ga subunit with GTP in the presence of Gβγ, causing the dissociation of the Ga subunit from the Gβγ dimer to form two functional units (Ga and Gβγ). Both Ga and Gβγ subunits signal to various cellular signaling pathways. Based on the sequence and functional homologies, G proteins are grouped into four families: Gs, Gi, Gq, and G12.

Gαi family (including Gαz) is the largest family of G proteins. They relay signals from many GPCRs to regualte various biological functions. There were no direct methods to measure the activation of Gαz proteins by receptors (until this assay kit). Most reports used one of the downstream pathway, i.e. the inhibition of adenylyl cyclases, as a readout.

NewEast Biosciences Gαz Activation Assay Kit provides a direct measurement of the activation of Gαz proteins. This is a simple and fast tool to monitor the activation of Gαz. Each kit provides sufficient quantities to perform 20 assays.

NewEast Biosciences Gαz Activation Assay Kit is based on the monoclonal antibody specifically recognizing the active GTP-bound Gαz proteins. This monoclonal antibody has much lower affinity towards the inactive Gαz proteins. Therefore, after activation by receptor signals, active GTP-bound Gαz proteins could be immunoprecipitated by this monoclonal antibody and further quantified by western blot with another anti- Gαz antibody.

 

Assay Principle