人E钙粘着蛋白/上皮性钙黏附蛋白(E-Cad)ELISA试剂盒kit价格
英文名称: Ecadherin/ epithelialcadherin(E-Cad)ELISA kitkit price
型号:null    抗体货号: YM-S0021
价格:请致电:010-57128832,18610462672
品牌: 国产/进口    产品商标: yuanmu

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sure the cGMP levels, either from cell extracts or from in vitro guanylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cGMP in cell extracts or in in vitro guanylyl cyclase assays will competitively bind to the monoclonal anti-cGMP antibody in the presence of fixed amounts of cGMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cGMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cGMP in samples. The measured optical density is used to calculate the concentration of cGMP in samples based on the curve from the cGMP standards.

 

 

Publications:
1.  Stimulation of guanylyl cyclase-D by bicarbonate
     Biochemistry, 2009
2.  Role of Sulfhydryl-Dependent Dimerization of Soluble Guanylyl Cyclase In Relaxation of Porcine Coronary Artery to Nitric Oxide
     Oxford Journals-Cardiovascular Research, 2011
3.  Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish
     Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2010
4.  Feedback Control Through cGMP-Dependent Protein Kinase Contributes to Differential Regulation and Compartmentation of cGMP in Rat Cardiac Myocytes
Circulation Research, 2010
5.  Pressure-Overload–Induced Subcellular Relocalization/Oxidation of Soluble Guanylyl Cyclase in the Heart Modulates Enzyme Stimulation
Circulation Research. 2012
6.  Inhibition of Striatal Soluble Guanylyl Cyclase-cGMP Signaling Reverses Basal Ganglia Dysfunction and Akinesia in Experimental Parkinsonism
PLoS ONE, 2011
7.  Sulfhydryl-dependent dimerization of soluble guanylyl cyclase modulates the relaxation of porcine pulmonary arteries to nitric oxide
Ye, L. Liu, J. Liu, H. Ying, L. Dou, D. Chen, Z. Xu, X. Raj, J. U. Gao, Y. 2012
8.  Natriuretic peptides block synaptic transmission by activating phosphodiesterase 2A and reducing presynaptic PKA activity
Hu, F. Ren, J. Zhang, J. E. Zhong, W. Luo, M. 2012
9.  Molt-inhibiting hormone from Chinese mitten crab (Eriocheir sinensis): Cloning, tissue expression and effects of recombinant peptide on ecdysteroid secretion of YOs
Zhang, Y. Sun, Y. Liu, Y. Geng, X. Wang, X. Wang, Y. Sun, J. Yang, W. 2011