Anti-Ras (Q61L) Mouse Monoclonal Antibody
英文名称：  Anti-Ras (Q61L) Mouse Monoclonal Antibody
型号:null    抗体货号： 26193
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Ras (Q61L) [26193]
[100 µL]		  

Catalog Number:  26193
Gene Symbol:  RAS (Q61L)
Description:   Anti-Ras (Q61L) Mouse Monoclonal Antibody
Background:   Ras (Q61L) is one type of Ras missence mutations at codon 61,Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation,and cell survival. Ras Q61L mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of Q61L, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA 1:100-1:5000
WB 1:100-1:1000
IF 1:50-1:100
IHC 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes Ras (Q61L), but not wild type Ras protein from vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), Ras Q61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras Q61L mouse monoclonal antibody (Cat. #26193).
Immunofluorescence:  
Immunofluorescence of cells expressing Ras proteins with anti-Ras (Q61L) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61H) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with anti-Ras (Q61L) monoclonal antibody (Cat. #26193).
 

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136