美国产品商标: neweastbioBrand,null 抗体货号： 26478Item#, Anti-RAS (A146P) Mouse Monoclonal Antibody<br>英文名称： Anti-RAS (A146P) Mouse Monoclonal Antibody

Ras (A146P) [26478]
[100ul]

Catalog Number: 26478

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS (A146P) Mouse Monoclonal Antibody

Background: The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.

Applications: ELISA, IF, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
IF: 1:50-1:100
IHC: 1:50-1:100

Concentration: 1 mg/ml

Host: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype:

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes A146P mutant, but not wild-type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunofluorescence:

Immunofluorescence of cells expressing KRas proteins with anti-KRas (A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRas (A146P) plasmid , pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146V) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRas (A146P) monoclonal antibody (Cat. #26478).

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)

2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136