Anti-Ras (G13D) Mouse Monoclonal Antibody
英文名称：  Anti-Ras (G13D) Mouse Monoclonal Antibody
型号:null    抗体货号： 26036
价格:请致电：010-57128832,18610462672
品牌: 美国    产品商标: neweastbio

 

Ras(G13D) [26038]
[100 µL]		  

Catalog Number:  26038
Gene Symbol:  RAS (G13D)
Description:   Anti-Ras (G13D) Mouse Monoclonal Antibody
Background:   Ras (G13D) is one type of Ras missense mutations at the codon 13. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G13D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
Immunogen:    A synthetic peptide from the internal region of Ras which includes the mutation of G13D, human origin.
Applications:   ELISA, WB, IF, IHC
Recommended Dilutions:   
ELISA: 1:100-1:3000
WB: 1:200-1:1000
IF: 1:50-1:100
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  RasG13D antibody recognizes RasG13D proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant Ras WT and G13D proteins with anti-Ras(G13D) monoclonal antibody. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G13D (lane 2, Cat # 10155) proteins were loaded and blotted with anti-Ras(G13D) mouse monoclonal antibody (Cat # 26038) (top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021).
Immunofluorescence:  
Immunofluorescence of cells expressing RAS proteins with anti-RAS (G13D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G13D) plasmid,pCDNA3-GFP-Ras(WT)plasmid, pCDNA3-GFP-Ras(G13A) plasmid,pCDNA3-GFP-Ras(G13V) plasmid, pCDNA3-GFP-Ras(G13C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G13S)plasmid, then fixed and stained with anti-Ras(G13D) monoclonal antibody (Cat. # 26038).
 

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
    J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136