美国产品商标: neweastbioBrand,null 抗体货号： 26195Item#, Anti-Ras (Q61H) Mouse Monoclonal Antibody<br>英文名称： Anti-Ras (Q61H) Mouse Monoclonal Antibody

Ras (Q61H) [26195]
[100ul]

Catalog Number: 26195

Gene Symbol: RAS

Description: Anti-Ras (Q61H) Mouse Monoclonal Antibody

Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras Q61H mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of Q61H, human origin

Applications: ELISA, WB, IF, IHC

Recommended Dilutions:
ELISA 1:100-1:5000
WB 1:100-1:2000
IF 1:50-1:100
IHC 1:50-1:100

Concentration: 2 mg/ml

Host: mouse

Format: Liquid

Clonality: Monoclonal

Isotype: Unknown

Purity: Purified from ascites

Storage buffer:

Preservative: no

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes RAS (Q61H) mutated, but not wild-type Ras proteins of vertebrates.

Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Western blot:

Western blot analysis of recombinant Ras proteins. Equal amount of purified Ras Q61H (lane 1), Ras Q61L (lane 2), Ras Q61R (lane 3), kRas WT (lane 4), hRas WT (lane 5), then were loaded and blotted with anti-Ras Q61H mouse monoclonal antibody (Cat. #26195).

Immunofluorescence:

Immunofluorescence of cells expressing Ras proteins with anti-Ras (Q61H) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras (Q61H) plasmid, pCDNA3-GFP-Ras (WT) plasmid, pCDNA3-GFP-Ras (Q61L) plasmid, pCDNA3-GFP-Ras (Q61R) plasmid or pCDNA3-GFP-Ras (Q61K) plasmid, then fixed and stained with anti-Ras (Q61H) monoclonal antibody (Cat. #26195).

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
Gene Therapy 21, 888-896 (October 2014)

2. Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a caecal adenocarcinoma and contiguous tubulovillous adenoma
J Clin Pathol. 2015 Aug;68(8):657-60. doi: 10.1136